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Phytosterols Elevation in Bamboo Shoot Residue through Laboratorial Scale Solid-State Fermentation Using Isolated Aspergillus niger CTBU
Zheng, X. X.1; Chen, R. S.1; Shen, Y.2; Yin, Z. Y.1
2014-04-01
摘要Aspergillus niger CTBU isolated from local decayed bamboo shoot residue was employed to solid-state fermentation (SSF) of bamboo shoot residue to elevate the content of phytosterols. Strain acclimatization was carried out under the fermentation condition using bamboo shoot as substrate for fermentation performance improvement. The optimal fermentation temperature and nitrogen level were investigated using acclimatized strain, and SSF was carried out in a 500-ml Erlenmeyer flask feeding 300-mg bamboo shoot residue chips under the optimal condition (33 A degrees C and feeding 4 % urea), and 1,186 mg (100 g)(-1) of total phytosterol was attained after 5-day fermentation, in comparison, only 523 mg (100 g)(-1) of phytosterol was assayed in fresh shoots residue. HPLC analysis of the main composition of total phytosterols displays that the types of phytosterols and composition ratio of main sterols keep steady. This laboratorial scale SSF unit could be scaled up for raw phytosterols production from discarded bamboo shoot residue and could reduce its cost.
关键词Solid-state fermentation Phytosterol Bamboo shoot residue Aspergillus niger
DOI10.1007/s12010-014-0821-6
发表期刊APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
卷号172期号:8页码:4078-4083
通讯作者Zheng, XX (reprint author), Chongqing Technol & Business Univ, Sch Environm & Biol Engn, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China.
收录类别SCI
WOS记录号WOS:000334433200029
语种英语