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Manure fertilization alters the population of ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea in a paddy soil
Wang, Yu1,2; Zhu, Guibing2; Song, Liyan1; Wang, Shanyun2,3; Yin, Chengqing2
2014-03-01
摘要Manure fertilizers are widely used in agriculture and highly impacted the soil microbial communities such as ammonia oxidizers. However, the knowledge on the communities of archaeal versus bacterial ammonia oxidizers in paddy soil affected by manure fertilization remains largely unknown, especially for a long-term influence. In present work, the impact of manure fertilization on the population of ammonia oxidizers, related potential nitrification rates (PNRs) and the key factors manipulating the impact were investigated through studying two composite soil cores (long-term fed with manure fertilization versus undisturbed). Moreover, soil incubated with NH4+ for 5 weeks was designed to verify the field research. The results showed that the copy numbers of bacterial amoA gene in the manure fed soil were significant higher than those in the unfed soil (p<0.05), suggesting a clear stimulating effect of long-term manure fertilization on the population of ammonia-oxidizing bacteria (AOB). The detected PNRs in the manure fed soil core (14-218nmolL(-1)Ng(-1)h(-1)) were significant higher than those in the unfed soil core (5-72nmolL(-1)Ng(-1)h(-1); p<0.05). Highly correlations between the PNRs and the bacterial amoA gene copies rather than archaeal amoA gene were observed, indicating strong nitrification capacity related to bacterial ammonia oxidizers. The NH4+-N significantly correlated to the abundance of AOB (p<0.01) and explained 96.1% of the environmental variation, showing the NH4+-N was the main factor impacting the population of AOB. The incubation experiment demonstrated a clear increase of the bacterial amoA gene abundance (2.0x10(6) to 8.4x10(6)g(-1)d.w.s. and 1.6x10(4) to 4.8x10(5)g(-1)d.w.s.) in both soil but not for the archaeal amoA gene, in agreement with the field observation. Overall, our results suggested that manure fertilization promoted the population size of bacterial ammonia oxidizers rather than their archaeal counterparts whether in long-term or short-term usage and the NH4+-N was the key impact factor.
关键词Ammonia-oxidizing archaea Ammonia-oxidizing bacteria Manure fertilization Paddy soil
DOI10.1002/jobm.201200671
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发表期刊JOURNAL OF BASIC MICROBIOLOGY
ISSN0233-111X
卷号54期号:3页码:190-197
通讯作者Zhu, GB (reprint author), Chinese Acad Sci, State Key Lab Environm Aquat Qual, Res Ctr Ecoenvironm Sci, POB 2871,Shuangqing Rd, Beijing 100085, Peoples R China.
收录类别SCI
WOS记录号WOS:000332082500004
语种英语