KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Investigation of the adsorption behavior of BSA with tethered lipid layer-modified solid-state nanopores in a wide pH range | |
Tian, Haibing1,2; Xie, Wanyi1,2 ; He, Shixuan1; Zhou, Daming1; Fang, Shaoxi1; Liang, Liyuan1,2; Wang, Deqiang1,2
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| 2019-05-19 | |
| 摘要 | Nanopore technology was introduced for the study of the dynamic interactions between bovine serum albumin (BSA) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) phospholipids based on a modified nanopore. The results reveal that the interaction mechanism between DOPE and BSA is affected by the pH of the subphase. Far above the BSA isoelectric point (pH > 7), a weaker hydrophobic interaction and stronger electrostatic repulsion exist between the DOPE and BSA molecules. At pH = 7, the BSA structure nearly does not change, and the interaction is weak. At pH 5 and pH 6, BSA is marginally affected by the adsorption interaction, and below pH 5, the DOPE film becomes disordered, so there is a strong repulsive force interaction between the BSA and DOPE. |
| DOI | 10.1039/c9ra00698b |
| 发表期刊 | RSC ADVANCES
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| ISSN | 2046-2069 |
| 卷号 | 9期号:27页码:15431-15436 |
| 收录类别 | SCI |
| WOS记录号 | WOS:000470161800037 |
| 语种 | 英语 |
