KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies | |
Yun, Wen1 ; Wu, Hong1; Liu, Xingyan1; Zhong, Haixia1; Fu, Min1; Yang, Lizhu2; Huang, Yu3
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| 2018-02-01 | |
| 摘要 | A novel fluorescent and colorimetric strategy with enzyme-free dual amplification was developed for ultra-sensitively detection of uranyl in river waters samples. The enzyme-strand recycling and hairpin catalytic assembly (HCA) reaction were used as dual enzyme-free amplification strategies. Three hairpins labeled with fluorophore can absorb on the surface of gold nanoparticles (AuNPs) to prevent them from aggregation in salt solution and quench the fluorescent signal simultaneously. In the presence of UO22+, the HCA reaction between hairpins was induced to form rigid DNA triangles, resulting in the aggregation of AuNPs with a red to blue color change and a "turn-on" fluorescent signal. The limit of detection was as low as 0.1 pM. Importantly, this strategy provided colorimetric and fluorescent dual signals for lower false-positives and higher anti-interference ability. It was successfully used for detection of uranyl in real waters samples. (C) 2017 Elsevier B.V. All rights reserved. |
| 关键词 | DNAzyme Gold nanoparticles Fluorescent Colorimetric Dual amplification |
| DOI | 10.1016/j.snb.2017.08.205 |
| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| 卷号 | 255页码:1920-1926 |
| 通讯作者 | Yun, W (reprint author), Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China. ; Yang, LZ (reprint author), Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000414319900089 |
| 语种 | 英语 |
