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Graphene Nanoplatelets-Based Aptamer Biochip for the Detection of Lipocalin-2
Matassan, Nur Diyanah1; Rizwan, Mohammad1,2; Mohd-Naim, Noor Faizah1,3; Tlili, Chaker4; Ahmed, Minhaz Uddin1
2019-11-01
摘要An electrochemical label-free aptasensor platform was developed by using amine-functionalized aptamer that was incorporated onto screen-printed carbon electrode (SPCE) modified with graphene nanoplatelets (GNPs) for the detection of lipocalin-2 (LCN-2) protein. The aptamers were covalently bound to the GNPs surface via 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry and were used as the molecular recognition element for the target protein. Successful interaction between aptamer and LCN-2 protein was ascertained via electrochemical probe, ferro-, and ferri-cyanide {Fe(CN)(6)(3-/4-)} and monitored by means of square wave voltammetry (SWV) whilst layer-by-layer fabrication study was done using cyclic voltammetry (CV). The developed LCN-2 aptasensor achieved sub-picomolar detection of the protein, with the detection limit determined as 0.07 pg ml(-1), and a dynamic linear range of 0.1 to 10 pg ml(-1). Fabricated aptasensor successfully demonstrated high selectivity, stability, and reproducibility as well as displaying potential for the in vitro detection of LCN-2 in real serum samples.
关键词Electrodes Proteins Graphene Biosensors Immune system Electrochemical aptasensor graphene nanoplatelets lipocalin screen-printed carbon electrode voltammetry
语种英语
卷号19
期号21
DOI10.1109/JSEN.2019.2927139
会议(录)名称IEEE SENSORS JOURNAL
页码9592-9599
通讯作者Ahmed, Minhaz Uddin(minhaz.ahmed@ubd.edu.bn)
收录类别SCI
会议地点New Delhi, India
会议日期October 28, 2018 - October 31, 2018
出版者IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC