KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System | |
Bian, Wan-Ping ; Chen, Yan-Ling; Luo, Juan-Juan; Wang, Chao; Xie, Shao-Lin; Pei, De-Sheng
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| 2019-04-01 | |
| 摘要 | The mitochondria DNA (mtDNA) editing tool, zinc finger nucleases (ZFNs), transcription activator-like effector nuclease (TALENs), and clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system, is a promising approach for the treatment of mtDNA diseases by eliminating mutant mitochondrial genomes. However, there have been no reports of repairing the mutant mtDNA with homologous recombination strategy to date. Here, we show a mito-CRISPR/Cas9 system that mito-Cas9 protein can specifically target mtDNA and reduce mtDNA copy number in both human cells and zebrafish. An exogenous single-stranded DNA with short homologous arm was knocked into the targeting loci accurately, and this mutagenesis could be steadily transmitted to F-1 generation of zebrafish. Moreover, we found some major factors involved in nuclear DNA repair were upregulated significantly by the mito-CRISPR/Cas9 system. Taken together, our data suggested that the mito-CRISPR/Cas9 system could be a useful method to edit mtDNA by knock-in strategy, providing a potential therapy for the treatment of inherited mitochondrial diseases. |
| 关键词 | mito-CRISPR/Cas9 mitochondria mtDNA editing ssDNA zebrafish |
| DOI | 10.1021/acssynbio.8b00411 |
| 发表期刊 | ACS SYNTHETIC BIOLOGY
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| ISSN | 2161-5063 |
| 卷号 | 8期号:4页码:621-632 |
| 收录类别 | SCI |
| WOS记录号 | WOS:000465487100002 |
| 语种 | 英语 |
