中国科学院重庆绿色智能技术研究院机构知识库

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	Soil microhabitats mediate microbial response in organic reduced tillage cropping
	Puerta, Viviana Loaiza1; Pereira, Engil Pujol1,2; Huang, Ping1,3; Wittwer, Raphael4; Six, Johan1
	2019-05-01
摘要	We aimed to understand how crop practices such as tillage, crop management and cover crop use affect aggregate microhabitats for nitrogen (N)-cycling microorganisms. We quantified the abundance of five N-cycling functional genes: two genes related to ammonia oxygenation (amoA from archaea and bacteria), two nitrite reducers (nirS and nirK) and one for nitrous oxide reduction (nosZ) as well as total eubacteria (16S) in two soil aggregate fractions. These fractions were total (occluded and non-occluded) microaggregates (tMi) and total silt and clay (tS + C) from a field trial under conventional and organic crop management combined with intensive or conservation tillage and four different cover crops. Although we found no clear associations between abundances of different functional genes in aggregate fractions, abundance of nosZ and amoA AOA changed almost exclusively in the tMi, suggesting that this fraction may mediate soil microbial response to crop management practices. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the nosZ gene indicated that aggregate fractions host different microbial communities, therefore providing distinct microhabitats.
关键词	Conservation tillage Cover crops Nitrogen Gene abundance nosZ amoA
DOI	10.1016/j.apsoil.2019.01.009
发表期刊	APPLIED SOIL ECOLOGY
ISSN	0929-1393
卷号	137页码:39-48
通讯作者	Puerta, Viviana Loaiza(viviana.loaiza@usys.ethz.ch)
收录类别	SCI
WOS记录号	WOS:000460377600005
语种	英语