中国科学院重庆绿色智能技术研究院机构知识库

QQ客服 官方微博 反馈留言

	Label-free identification of trace microcystin-LR with surface-enhanced Raman scattering spectra
	He, Shixuan1,2; Xie, Wanyi1; Fang, Shaoxi1; Zhou, Daming1; Djebbi, Khouloud1; Zhang, Zhiyou2; Du, Jinglei2; Du, Chunlei1,2; Wang, Deqiang1
	2019-04-01
摘要	The analysis of trace microcystin-LR (MC-LR) plays important roles in environmental fields, especially in monitoring domestic water quality and safety, since it has particularly harmful effect on wild and domestic animals as well as humans at low doses. Herein, we combine confocal Raman spectroscopy with SERS-AG substrate to characterize the "fingerprint" information of MC-LR directly. High sensitivity of SERS-AG substrates was verified by utilizing the probe molecule Rhodamine 6 G. Mapping spectra demonstrated good reproducibility of MC-LR identification with label-free surface-enhanced Raman scattering (SERS) strategy. Differences between SERS spectra of MC-LR and R6G, microcystin-RR were evaluated by calculating their scores and loading weights with an unsupervised exploratory principal component analysis method. Then, relationship between Raman intensities and concentrations was preliminary analyzed with SERS spectra of MC-LR and the lowest concentration of MC-LR identification was 10(-6) mg L-1 while using SERS-AG substrate. Thereafter, 68.6% quantitative recovery of 10(-3) mg L-1 MC-LR in tap water samples was obtained by the proposed label-free SEAS method. These results showed that confocal Raman spectroscopy with label-free surface-enhanced Raman scattering strategy can handle the identification of trace MC-LR for monitoring water quality and safety worldwide in future.
关键词	Microcystin-LR Surface-enhanced Raman scattering Principal component analysis
DOI	10.1016/j.talanta.2018.11.072
发表期刊	TALANTA
ISSN	0039-9140
卷号	195页码:401-406
收录类别	SCI
WOS记录号	WOS:000456762000052
语种	英语