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	Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures
	Tang, Mingjie1; Zhang, Mingkun1; Yan, Shihan1; Xia, Liangping1; Yang, Zhongbo1; Du, Chunlei1; Cui, Hong-Liang1,2; Wei, Dongshan1
	2018-01-24
摘要	DNA oligonucleotides with a 5-base mutation at the 3'-terminus were investigated by terahertz (THz) spectroscopy in a marker-free manner. The four single-stranded oligonucleotides with 17nt have been detected with specificity on a microfluidic chip, and corroborated by spectral measurements with split-ring resonators. The number of hydrogen bonds formed between the oligonucleotide and its surrounding water molecules, deemed a key contribution to the THz absorption of biological solutions, was explored by molecular dynamics simulations to explain the experimental findings. Our work underlies the feasibility of THz spectroscopy combined with microstructures for marker-free detection of DNA, which may form the basis of a prospective diagnostic tool for studying genic mutation.
DOI	10.1371/journal.pone.0191515
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	13期号:1页码:12
收录类别	SCI
WOS记录号	WOS:000423412500055
语种	英语