KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Rapid discrimination of DNA sequences using an engineered nanopore | |
Ying, Cuifeng1,3; Feng, Yanxiao1; Wang, Deqiang1 ; Guan, Xiyun2; Zhou, Wenyuan3; Tian, Jianguo3; Zhang, Yuechuan4
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| 2015 | |
| 摘要 | Nanopores have been used for the development of biosensors for various substances and are currently under intense investigation as a potential tool for the entire genome sequencing. It has been demonstrated that an α-hemolysin protein pore covalently attached with a β-cyclodextrin adapter can differentiate single bases[1]; furthermore, single base discrimination could be achieved when a single-stranded DNA (ssDNA) is immobilized in the α-hemolysin pore[2]. Here, we show that when an unlabelled/unmodified double-stranded DNA (dsDNA) translocates through a properly engineered α-hemolysinnanopore, a sequence of sub-state blockage events is observed. Since different sequences of sub-state modulation events are produced by different DNA strands, they offer the potential as characteristic fingerprints to identify and discriminate the precise sequences of dsDNA samples. © 2015 IEEE. |
| 语种 | 英语 |
| DOI | 10.1109/3M-NANO.2015.7425464 |
| 会议(录)名称 | 5th International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale, 3M-NANO 2015
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| 页码 | 272-277 |
| 收录类别 | EI |
| 会议地点 | Changchun, China |
| 会议日期 | October 5, 2015 - October 9, 2015 |
