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Rapid and label-free detection and assessment of bacteria by terahertz time-domain spectroscopy
Yang, Xiang1; Wei, Dongshan2; Yan, Shihan2; Liu, Yueping1; Yu, Shu1; Zhang, Mingkun2; Yang, Zhongbo2; Zhu, Xiaoyan1; Huang, Qing1; Cui, Hong-Liang2,3
2016-10-01
摘要Here we demonstrated the potential and applicability of terahertz (THz) spectroscopy to detect four commonly found bacteria in the infectious diseases. Besides the different spectral characteristics between bacterial species, THz absorption differences for living bacteria, dead bacteria and bacterial powder of the same species were also investigated. Our results revealed that small differences in water contents between bacterial cells account for distinct discrepancies of the absorption coefficients, which can be used for bacterial species identification. Furthermore, living and dead bacteria showed different absorption coefficients as a result of their different hydration levels, suggesting that THz spectroscopy can be used to rapidly assess the living state of bacteria under test. Our results clearly demonstrated the ability of THz spectroscopy for time-saving and label-free detection of bacteria with minimal sample preparation, potentially to be utilized for point-of-care tests in the near future. [GRAPHICS] Schematic representation of bacterial detection by THz spectroscopy. Different bacteria have distinctive absorption coefficients as a result of their different water contents.
关键词bacterial analysis laboratory medicine terahertz time-domain spectroscopy absorption coefficient
DOI10.1002/jbio.201500270
发表期刊JOURNAL OF BIOPHOTONICS
ISSN1864-063X
卷号9期号:10页码:1050-1058
通讯作者Fu, WL (reprint author), Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China. ; Cui, HL (reprint author), Chinese Acad Sci, Chongqing Key Lab Multiscale Mfg Technol, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China. ; Cui, HL (reprint author), Jilin Univ, Coll Instrumentat Sci & Elect Engn, Changchun 130061, Jilin, Peoples R China.
收录类别SCI
WOS记录号WOS:000388372800008
语种英语