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Endocytic Fluorescence of Nanodiamond in Living Hela Cells Determined by Microscopy Imaging Techniques
Zheng, Mei-Ling1,2; Lei, Ying1,2,5; Zheng, Yong-Chao1,2,5; Xu, Tao4,5; Zhi, Jin-Fang4; Zhao, Zhen-Sheng1,2; Duan, Xuan-Ming1,2,3
2016-07-01
摘要We have prepared the carboxylate nanodiamond (ND-COOH) and demonstrated the enhanced endocytic fluorescence in living Hela cells by using confocal fluorescence microscopy imaging and transmission electron microscopy (TEM) techniques. The fluorescence enhancement of ND-COOH is observed during the staining dynamics of living cells using confocal fluorescence microscopy. Furthermore, the intracellular localization of ND-COOH in endocytic cells is verified by TEM and the three-dimensional (3D) optical sectioning image of confocal fluorescence microscopy. The low cytotoxicity and high photostability have also been clearly demonstrated. The characterization and the analysis of microscopy provide the unique direct evidence that the ND-COOH can enter living cells though the endocytosis process and exhibits strong endocytic fluorescence. This study would open up new avenues towards the application of functional ND in living cell labelling or drug delivery system.
关键词Fluorescence Enhancement Nanodiamond Microscopy Living Cell Imaging
DOI10.1166/jnn.2016.11307
发表期刊JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
ISSN1533-4880
卷号16期号:7页码:6748-6754
通讯作者Zheng, ML ; Duan, XM (reprint author), Chinese Acad Sci, Lab Organ NanoPhoton, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. ; Zheng, ML ; Duan, XM (reprint author), Chinese Acad Sci, Key Lab Funct Crystals & Laser Technol, Tech Inst Phys & Chem, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. ; Duan, XM (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Shuitu Technol Dev Zone, 266 Fangzheng Ave, Chongqing 400714, Peoples R China.
收录类别SCI
WOS记录号WOS:000387100400018
语种英语