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Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation
Wang, Huabin1,2,4; Wilksch, Jonathan J.3; Chen, Ligang1; Tan, Jason W. H.3; Strugnell, Richard A.3; Gee, Michelle L.2
2017-01-20
摘要The surface polymers of bacteria determine the ability of bacteria to adhere to a substrate for colonization, which is an essential step for a variety of microbial processes, such as biofilm formation and biofouling. Capsular polysaccharides and fimbriae are two major components on a bacterial surface, which are critical for mediating cell-surface interactions. Adhesion and viscoelasticity of bacteria are two major physical properties related to bacteria surface interactions. In this study, we employed atomic force microscopy (AFM) to interrogate how the adhesion work and the viscoelasticity of a bacterial pathogen, Klebsiella pneumoniae, influence biofilm formation. To do this, the wild-type, type 3 fimbriae-deficient, and type 3 fimbriae-overexpressed K. pneumoniae strains have been investigated in an aqueous environment. The results show that the measured adhesion work is positively correlated to biofilm formation; however, the viscoelasticity is not correlated to biofilm formation. This study indicates that AFM-based adhesion measurements of bacteria can be used to evaluate the function of bacterial surface polymers in biofilm formation and to predict the ability of bacterial biofilm formation.
DOI10.1021/acs.langmuir.6b03764
发表期刊LANGMUIR
ISSN0743-7463
卷号33期号:1页码:100-106
通讯作者Wang, HB (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China. ; Wang, HB ; Gee, ML (reprint author), Univ Melbourne, Sch Chem, Parkville, Vic 3010, Australia. ; Wang, HB (reprint author), Chinese Acad Sci, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China.
收录类别SCI
WOS记录号WOS:000391898100012
语种英语