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Nondegenerate two-photon absorption properties of a newly synthesized carbazole derivative
Chen, Shu1,2,4; Zheng, Yong-Chao1,2,5; Zheng, Mei-Ling1,2; Dong, Xian-Zi1,2; Jin, Feng1,2; Zhao, Zhen-Sheng1,2; Duan, Xuan-Ming3
2017-01-14
摘要A two-color two-photon laser scanning microscope (2c2pLSM) has been developed for fluorescence imaging in tissues through the nondegenerate two-photon excitation of fluorescent proteins. However, fluorescent probes have not been widely designed and applied to 2c2pLSM. Here, we have synthesized a new carbazole derivative 3,6-bis(pyridin-4-ylethynyl)-9-methyl-carbazole (BEMC), and investigated its nondegenerate two-photon absorption (ND-TPA) properties. Nondegenerate two-photon excited fluorescence (ND-TPEF) and ND-TPA cross-sections excited by a constant pump wavelength of 800 nm and several probe wavelengths from 485 nm to 650 nm were studied based on the femtosecond pump-probe technique. The fluorescence intensity is linearly dependent on each laser intensity of the two beams, and the dependence of ND-TPEF intensity on time delay between the two lasers is in good agreement with the cross-correlation of the pulse widths of the two lasers. Compared with the corresponding degenerate two-photon absorption (D-TPA) cross-sections for transition to the same excited state via the D-TPA pathway, the ND-TPA cross-sections exhibit at least twofold larger values than the D-TPA case, which is attributed to the effect of intermediate state resonance enhancement (ISRE). This study indicates that the newly synthesized carbazole derivative would serve as a promising fluorescent probe in a two-color two-photon laser scanning microscope (2c2pLSM).
DOI10.1039/c6tc04676b
发表期刊JOURNAL OF MATERIALS CHEMISTRY C
ISSN2050-7526
卷号5期号:2页码:470-475
通讯作者Zheng, ML (reprint author), Chinese Acad Sci, Lab Organ NanoPhoton, Tech Inst Phys & Chem, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. ; Zheng, ML (reprint author), Chinese Acad Sci, Lab Organ NanoPhoton, Lab Bioinspired Smart Interface Sci, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. ; Duan, XM (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, 266 Fangzheng Ave, Chongqing 400714, Peoples R China.
收录类别SCI
WOS记录号WOS:000392813800027
语种英语