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Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE
Liu, Yingbao1,2,4; Yang, Xiaobing1,2; Yin, Yajie5; Lin, Jinshui1,2; Chen, Can1,2; Pan, Junfeng1,2; Si, Meiru3; Shen, Xihui1,2
2016
摘要Mycothiol (MSH) plays a major role in protecting cells against oxidative stress and detoxification from a broad range of exogenous toxic agents. In the present study, we reveal that intracellular MSH contributes significantly to the adaptation, to acidic conditions in the model organism Corynebacterium glutamicum. We present evidence that MSH confers C. glutanzicum with the ability to adapt to acidic conditions by maintaining pH(i) homeostasis, scavenging reactive oxygen species (ROS), and protecting methionine synthesis by the S-mycothiolation modification of methionine synthase (MetE). The role of MSH in acid adaptation was further confirmed by improving the acid tolerance of C. glutamicum by overexpressing the key MSH synthesis gene mshA. Hence, our work provides insights into a previously unknown, but important, aspect of the C. glutamicum cellular response to acid stress. The results reported here may help to understand acid tolerance mechanisms in acid sensitive bacteria and may open a new avenue for improving acid resistance in industry strains for the production of bio-based chemicals from renewable biomass.
关键词acid stress Corynebacterium glutamicum mycothiol pH homeostasis S-mycothiolation
DOI10.2323/jgam.2016.02.001
发表期刊JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
ISSN0022-1260
卷号62期号:3页码:144-153
通讯作者Shen, XH (reprint author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. ; Si, MR (reprint author), Qufu Normal Univ, Coll Life Sci, Qufu 273165, Shandong, Peoples R China.
收录类别SCI
WOS记录号WOS:000381846300006
语种英语