KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Monitoring tetracycline through a solid-state nanopore sensor | |
Zhang, Yuechuan1,2; Chen, Yanling3 ; Fu, Yongqi1; Ying, Cuifeng2,4; Feng, Yanxiao2; Huang, Qimeng2; Wang, Chao3; Pei, De-Sheng3; Wang, Deqiang2
| |
| 2016-06-16 | |
| 摘要 | Antibiotics as emerging environmental contaminants, are widely used in both human and veterinary medicines. A solid-state nanopore sensing method is reported in this article to detect Tetracycline, which is based on Tet-off and Tet-on systems. rtTA (reverse tetracycline-controlled trans-activator) and TRE (Tetracycline Responsive Element) could bind each other under the action of Tetracycline to form one complex. When the complex passes through nanopores with 8 similar to 9 nanometers in diameter, we could detect the concentrations of Tet from 2 ng/mL to 2000 ng/mL. According to the Logistic model, we could define three growth zones of Tetracycline for rtTA and TRE. The slow growth zone is 0-39.5 ng/mL. The rapid growth zone is 39.5-529.7 ng/mL. The saturated zone is > 529.7 ng/mL. Compared to the previous methods, the nanopore sensor could detect and quantify these different kinds of molecule at the single-molecule level. |
| DOI | 10.1038/srep27959 |
| URL | 查看原文 |
| 发表期刊 | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| 卷号 | 6页码:7 |
| 收录类别 | SCI |
| WOS记录号 | WOS:000378074400001 |
| 语种 | 英语 |
