中国科学院重庆绿色智能技术研究院机构知识库

QQ客服 官方微博 反馈留言

	Biosensing of BCR/ABL fusion gene using an intensity-interrogation surface plasmon resonance imaging system
	Wu, Jiangling1; Huang, Yu1,2; Bian, Xintong; Li, DanDan1; Cheng, Quan1,3; Ding, Shijia1
	2016-10-15
摘要	In this work, a custom-made intensity-interrogation surface plasmon resonance imaging (SPRi) system has been developed to directly detect a specific sequence of BCR/ABL fusion gene in chronic myelogenous leukemia (CML). The variation in the reflected light intensity detected from the sensor chip composed of gold islands array is proportional to the change of refractive index due to the selective hybridization of surface-bound DNA probes with target ssDNA. SPRi measurements were performed with different concentrations of synthetic target DNA sequence. The calibration curve of synthetic target sequence shows a good relationship between the concentration of synthetic target and the change of reflected light intensity. The detection limit of this SPRi measurement could approach 10.29 nM. By comparing SPRi images, the target ssDNA and non-complementary DNA sequence are able to be distinguished. This SPRi system has been applied for assay of BCR/ABL fusion gene extracted from real samples. This nucleic acid based SPRi biosensor therefore offers an alternative high-effective, high-throughput label-free tool for DNA detection in biomedical research and molecular diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Surface plasmon resonance imaging Gold microarray DNA hybridization BCR/ABL fusion gene
DOI	10.1016/j.optcom.2016.05.035
URL	查看原文 ; 查看原文
发表期刊	OPTICS COMMUNICATIONS
ISSN	0030-4018
卷号	377页码:24-32
通讯作者	Ding, SJ (reprint author), Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.
收录类别	SCI
WOS记录号	WOS:000377397600004
语种	英语