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Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge
Wu, Boran1; Su, Lianghu2; Song, Liyan3; Dai, Xiaohu1; Chai, Xiaoli1
2017-02-01
摘要To characterize the transformation of extracellular proteins extracted from raw and enzymedisintegrated waste activated sludge (WAS), extracts of extracellular polymeric substances (EPS) were subject to isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis. 209 proteins were identified and categorized into three Gene Ontology classifications: "cellular components", "molecular function", and "biological processes". Most identified proteins originated from intracellular components, organelles, or cytoplasm, suggesting that cell decline and lysis represent the main sources of extracellular proteins in WAS. The major protein functions comprised "transporter binding activity", "macromolecule metabolic process", and "biosynthesis enzyme catalytic activity". In total, 15 proteins categorized as "membrane part" and "biological adhesion" and 10 as "organelle" were down-or up-regulated, respectively, implying that the enzyme-disintegration mainly induced WAS floc-structure disintegration via membrane structure and corresponding biological adhesion disruption. The proteomics study will provide valuable clues to better understand EPS changes associated with enzymatic treatment at molecular levels. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Waste activated sludge Extracellular polymeric substances (EPS) iTRAQ Proteomics
DOI10.1016/j.biortech.2016.11.041
发表期刊BIORESOURCE TECHNOLOGY
ISSN0960-8524
卷号225页码:75-83
通讯作者Chai, XL (reprint author), Tongji Univ, State Key Lab Pollut Control & Resource Reuse, Coll Environm Sci & Engn, 1239 Siping Rd, Shanghai 200092, Peoples R China.
收录类别SCI
WOS记录号WOS:000395693700008
语种英语