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The effect of light on the cellular stoichiometry of Chlorella sp. in different growth phases: implications of nutrient drawdown in batch experiments
Liu, Jing1; Li, Zhe2; Guo, Jing-song1,2; Xiao, Yan2; Fang, Fang1; Qin, Rong-cong2; Zhang, Ling-ling3
2017-02-01
摘要Identification of nutrient status is essential to Chlorella sp. cultivation for biodiesel production. Critical cellular elemental ratio is a commonly used parameter to assess nutrient status, but the definition of critical cellular elemental ratio is inconsistent, and the influence of light and growth phase on this ratio is still unclear. In this study, Chlorella sp. was batch cultured under different conditions of light and nutrient supply ratio. Nitrate and phosphate consumption in the medium and particulate cellular carbon (C), nitrogen (N), and phosphorus (P) contents were measured. We first obtained the critical nutrient supply ratio to assess nutrient limitation and then utilized this ratio to deduce the critical cellular elemental ratio. The critical nutrient supply ratio was significantly affected by light intensity, and was approximately 45, 15, and between 30 to 45 under light intensities of 50, 100, and 200 mu mol photons m(-2) s(-1), respectively. Critical cellular C/N/P ratios of Chlorella sp. at three light intensities of 50, 100, and 200 mu mol photons m(-2) s(-1) in exponential growth phase were C159.91-237.30N15.90-22.52P, C104.02-167.39N11.71-17.35P, and C144.30-243.66N12.84-19.84P, and in stationary growth phase the ratios were C201.47-406.14N16.55-32.67P, C125.05-298.44N10.12-24.24P, and C168.65-342.42N14.47-29.18P, respectively. These results supported the hypothesis that the critical cellular elemental ratio, which is used to predict nutrient status, varies and depends on light intensity and growth phase.
关键词Chlorella sp. Critical nutrient supply ratio Critical cellular elemental ratio Light intensity Growth phase
DOI10.1007/s10811-016-0962-9
发表期刊JOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
卷号29期号:1页码:123-131
通讯作者Li, Z (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China.
收录类别SCI
WOS记录号WOS:000396344800012
语种英语