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Application of Faecalibacterium 16S rDNA genetic marker for accurate identification of duck faeces
Sun, Da1; Duan, Chuanren1; Shang, Yaning1; Ma, Yunxia1; Tan, Lili1; Zhai, Jun2; Gao, Xu2; Guo, Jingsong3; Wang, Guixue1
2016-04-01
摘要The aim of this study was to judge the legal duty of pollution liabilities by assessing a duck faeces-specific marker, which can exclude distractions of residual bacteria from earlier contamination accidents. With the gene sequencing technology and bioinformatics method, we completed the comparative analysis of Faecalibacterium sequences, which were associated with ducks and other animal species, and found the sequences unique to duck faeces. Polymerase chain reaction (PCR) and agarose gel electrophoresis techniques were used to verify the reliability of both human and duck faeces-specific primers. The duck faeces-specific primers generated an amplicon of 141 bp from 43.3 % of duck faecal samples, 0 % of control samples and 100 % of sewage wastewater samples that contained duck faeces. We present here the initial evidence of Faecalibacterium-based applicability as human faeces-specificity in China. Meanwhile, this study represents the initial report of a Faecalibacterium marker for duck faeces and suggests an independent or supplementary environmental biotechnology of microbial source tracking (MST).
关键词Faecalibacterium Host-specific PCR assay Water pollution Duck
DOI10.1007/s11356-015-6024-z
发表期刊ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN0944-1344
卷号23期号:8页码:7639-7647
通讯作者Duan, CR ; Wang, GX (reprint author), Chongqing Univ, State & Local Joint Engn Lab Vasc Implants Chongq, Bioengn Coll, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing 630044, Peoples R China.
收录类别SCI
WOS记录号WOS:000374994600056
语种英语