KMS Chongqing Institute of Green and Intelligent Technology, CAS
Detection of ESAT-6 by a label free miniature immuno-electrochemical biosensor as a diagnostic tool for tuberculosis | |
Diouani, Mohamed Fethi1,2,6; Ouerghi, Oussama3,4; Refai, Amira2,4; Belgacem, Kamel1,4; Tlili, Chaker1,5; Laouini, Dhafer2,4; Essafi, Makram2,4 | |
2017 | |
摘要 | Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major virulent factor of Mtb, namely the early secreted antigenic target 6-kDa protein or ESAT-6. A label free electrochemical immunosensor using an anti-ESAT-6 monoclonal antibody as a bio-receptor is described herein. Anti-ESAT-6 antibodies were first covalently immobilized on the surface of a gold screen-printed electrode functionalized via a self-assembled thiol monolayer. Interaction between the bio-receptor and ESAT-6 antigen was evaluated by square wave voltammetry method using [F-e(CN)(6)](3-/4-) as redox probe. The detection limit of ESAT-6 antigen was 7 ng/ml. The immunosensor has also been able to detect native ESAT-6 antigen secreted in cell culture filtrates of three pathogenic strains of Mtb (CDC1551, H37RV and H8N8). Overall, this work describes an immune-electrochemical biosensor, based on ESAT-6 antigen detection, as a Useful diagnostic tool for tuberculosis. 2016 Elsevier B.V. All rights reserved. |
DOI | 10.1016/j.msec.2016.12.051 |
发表期刊 | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS |
ISSN | 0928-4931 |
卷号 | 74页码:465-470 |
收录类别 | SCI |
WOS记录号 | WOS:000397356900054 |
语种 | 英语 |