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	Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb2+ using molecular beacon and DNAzyme based amplification strategy
	Yun, Wen1; Cai, Dingzhou1; Jiang, JiaoLai1; Zhao, Pengxiang1; Huang, Yu2; Sang, Ge1
	2016-06-15
摘要	An enzyme-free and label-free colorimetric Pb2+ sensor based on DNAzyme and molecular beacon (MB) has been developed and demonstrated by recycle using enzyme strand for signal amplification. The substrate strand DNA (S-DNA) of DNAzyme could be converted into MB structure with base pairs of stem part at the both ends. The MB could hybridize with enzyme strand DNA (E-DNA) to form DNAzyme, and be activated and cleaved in the presence of Pb2+. The cleaved MB is much less stable, releasing from the DNAzyme as two product pieces. The product pieces of MB are flexible and could bind to unmodified AuNPs to effectively stabilize them against salt-induced aggregation. Then, the E-DNA is liberated to catalyze the next reaction and amplify the response signal. By taking advantage of repeated using of E-DNA, our proposed method exhibited high sensitive for Pb2+ detection in a linear range from 0.05 to 5 nM with detection limit of 20 pM by UV-vis spectrometer. Moreover, this method was also used for determination of Pb2+ in river water samples with satisfying results. Importantly, this strategy could reach high sensitivity without any modification and complex enzymatic or hairpins based amplification procedures. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Molecular beacon Gold nanoparticles DNAzyme Pb2+ detection
DOI	10.1016/j.bios.2016.01.053
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	80页码:187-193
通讯作者	Sang, G (reprint author), Sci & Technol Surface Phys & Chem Lab, POB 9-35, Jiangyou City 621908, Sichuan, Peoples R China. ; Huang, Y (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, 266 Fangzheng Ave,Shuitu Hitech Pk, Chongqing 400714, Peoples R China.
收录类别	SCI
WOS记录号	WOS:000372558500028
语种	英语