KMS Chongqing Institute of Green and Intelligent Technology, CAS
| Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish | |
Zhang, Jing-Hui1,2; Sun, Tai1 ; Niu, Aping1; Tang, Yu-Mei1; Deng, Shun1; Luo, Wei1; Xu, Qun2; Wei, Dapeng1; Pei, De-Sheng1
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| 2017-07-01 | |
| 摘要 | Graphene quantum dots (GQDs) has been widely used in enormous fields, however, the inherent molecular mechanism of GQDs for potential risks in biological system is still elusive to date. In this study, the outstanding reduced graphene quantum dots (rGOQDs) with the QY as high as 24.62% were successfully synthesized by the improved Hummers method and DMF hydrothermal treatment approach. The rGOQDs were N-doped photoluminescent nanomaterials with functional groups on the surface. The fluorescent bio-imaging was performed by exposing zebrafish in different concentrations of the as-prepared rGOQDs, and the distribution of rGOQDs was successfully observed. Moreover, the developmental toxicity and genotoxicity were evaluated to further investigate the potential hazard of rGOQDs. The result indicated that rGOQDs were responsible for the dose-dependent abnormalities on the development of zebrafish. Since the real-time polymerase chain reaction (RT-PCR) results showed that the expression of cyp1a was the highest expression in the selected genes and significantly up-regulated 8.49 fold in zebrafish, the perturbation of rGOQDs on aryl hydrocarbon receptor (AhR) pathway was investigated by using the Tg(cyp1a:gfp) zebrafish for the first time. The results demonstrated that rGOQDs significantly increased the green fluorescent protein (GFP) expression promoted by cyp1a in a dose-dependent manner, which was also further confirmed by the western blotting. This study offered an opportunity to reveal the potential hazards of in vivo bio-probes, which provided a valuable reference for investigating the graphene-based materials on the disturbance of AhR pathway in biological organisms. (C) 2017 Elsevier Ltd. All rights reserved. |
| 关键词 | AhR pathway Bio-imaging Reduced graphene oxide quantum dots Real-time PCR Zebrafish |
| DOI | 10.1016/j.biomaterials.2017.04.026 |
| 发表期刊 | BIOMATERIALS
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| ISSN | 0142-9612 |
| 卷号 | 133页码:49-59 |
| 收录类别 | SCI |
| WOS记录号 | WOS:000401716700006 |
| 语种 | 英语 |
