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Isolation of Extracellular Outer Membrane Vesicles (OMVs) from Escherichia coli Using EVscore47 Beads
Shi, Gongming1; Yang, Xiaohong2; Wang, Jikai3; Wei, Wenjing2; Hu, Kecui2; Huang, Xingyue2; Qiu, Yanfei2; He, Yun1
2024-04-01
摘要Outer membrane vesicles (OMVs) are attractive for biomedical applications based on their intrinsic properties in relation to bacteria and vesicles. However, their widespread use is hampered by low yields and purities. In this study, EVscore47 multifunctional chromatography microspheres were synthesized and used to efficiently isolate functional OMVs from Escherichia coli. Through this technology, OMV loss can be kept to a minimum, and OMVs can be harvested using EVscore47 at 11-fold higher yields and similar to 13-fold higher purity than those achieved by means of ultracentrifugation. Based on the results presented here, we propose a novel EVscore47-based isolation of OMVs that is fast and scalable.
关键词multifunctional chromatography microspheres purification outer membrane vesicles
DOI10.3390/molecules29081831
发表期刊MOLECULES
卷号29期号:8页码:14
通讯作者He, Yun(yun.he@cqu.edu.cn)
收录类别SCI
WOS记录号WOS:001211285000001
语种英语