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A multilevel core-shell structure Fe3O4@SiO2@InGaO@SiO2 magnetic particles for DNA extraction
Liu, Shaoxiang1,2,3; Ning, Shuhua4; Zhang, Kun2; Wu, Yutong2; Feng, Shuanglong2,3; Lu, Wenqiang1,2,3
2024-06-15
摘要DNA extraction is greatly significant in biological detection fields, including species identification, pathogen detection, environmental monitoring, biosafety, and food safety. Scientific research and practical application must conduct high-quality trace DNA separation, purification and extraction. Herein, a two-step strategy successfully prepares a multilevel core-shell structure Fe3O4@SiO2@InGaO@SiO2 magnetic particles for trace DNA extraction. The morphologies, structures, elemental compositions, components, and properties of the materials are systematically characterized. Fe3O4@SiO2@InGaO@SiO2 magnetic particles in the chaotropic salt solutions are used to directly target DNA binding due to the existence of Si-O-H bond. The maximum DNA adsorption rate can highly reach 83.1 % after 700 degrees C annealing in the air for 30 min, far exceeding the adsorption rate of commercial reagent kit, which is attributed to the increased specific surface area. The excellent result is conducive to rapid DNA extraction in practical applications, especially in daily criminal cases, further promoting social security and stability.
关键词DNA extraction Multilevel core-shell structure CVD technique
DOI10.1016/j.apsusc.2024.159823
发表期刊APPLIED SURFACE SCIENCE
ISSN0169-4332
卷号658页码:7
通讯作者Feng, Shuanglong(fengshuanglong@cigit.ac.cn) ; Lu, Wenqiang(luwq@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:001209703900001
语种英语