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Optical fiber surface plasmon resonance sensor using electroless-plated gold film for thrombin detection
Huang, Yu1,2; Pu, Xiaoyin1,2; Qian, Husun3; Chuang, Chin-Jung4; Dong, Shanshan5; Wu, Jiangling5; Xue, Jianjiang5; Cheng, Wei6; Ding, Shijia7; Li, Shengqiang8,9
2024-01-18
摘要This paper describes the simple and label-free detection of thrombin using optical fiber surface plasmon resonance (SPR) sensors based on gold films prepared by the cost-effective method of electroless plating. The plating conditions for simultaneously obtaining gold film on cylindrical core and end surfaces of an optical fiber suitable for measurement were optimized. The fabricated sensor exhibited a linear refractive index sensitivity of 2150 nm/RIU and 7.136 (a.u.)/RIU in the refractive index of 1.3329-1.3605 interrogated by resonance wavelength and amplitude methods respectively and a single wavelength monitoring method was proposed to investigate the sensing performance of this sensor. Polyadenine diblock and thiolated thrombin aptamers were immobilized on gold nanoparticles and gold films respectively to implement a sandwich optical fiber assay for thrombin. The developed optical fiber SPR sensors were successfully used in the determination of thrombin down to 0.56 nM over a wide range from 2 to 100 nM and showed good selectivity for thrombin, which indicated their potential clinical applications for biomedical samples.
关键词Optical fiber Surface plasmon resonance Electroless plating Biosensing
DOI10.1007/s00216-024-05150-x
发表期刊ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
页码15
通讯作者Huang, Yu(huangyu@cigit.ac.cn) ; Wu, Jiangling(wujiangling@hospital.cqmu.edu.cn) ; Li, Shengqiang(yixuelishengqiang@126.com)
收录类别SCI
WOS记录号WOS:001145225600003
语种英语