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Sequential Visual Sensing of H2O2 and GSH Based on Fluorescent Copper Nanoclusters Incorporated Eggshell Membrane
Liang, Mengna1; Yuan, Lili1; Shao, Congying1; Zheng, Xingqun2; Song, Qingchun3; Gu, Zhengrong3; Lu, Shun4
2023-08-15
摘要Digital imaging technology provides a unique advantage for the portable on-site detection of targeted analytes. In this work, copper nanoclusters (CuNCs) based on the eggshell membrane (ESM) were combined with the photograph-taking and color recognition functions of a smartphone to build an "ON-OFF-ON" detection platform, which realized the dual visual detection of hydrogen peroxide (H2O2) and glutathione (GSH). First, a red fluorescent nanocomposite membrane (GSH-CuNCs@ESM) was prepared by in situ synthesis with GSH as the protective and reducing agent. The physical characterization results confirmed its long fluorescence (FL) lifetime (23.77 mu s), high quantum yield (QY, 23.79%), large Stokes shift, and good stability. In addition, it is found that H2O2 has a specific response to the GSH-CuNCs@ESM, which can quench its FL. However, the FL of the quenched membrane was restored after it was put into the GSH solution. So, visual detection of H2O2 and GSH was developed based on this phenomenon integrating with a smartphone and color recognition functions. Consequently, the limit of detection (LOD) of H2O2 is as low as 63 nM when the concentration of H2O2 is between 0 and 100 mu M. The LOD of GSH is as low as 1.0 mu M when the concentration of GSH was between 0 and 800 mu M.
关键词Biomembranes Fluorescence Sensors Visualization Probes Stability analysis Image color analysis Copper nanoclusters (CuNCs) eggshell membrane (ESM) smartphone platform visual detection
DOI10.1109/JSEN.2023.3295173
发表期刊IEEE SENSORS JOURNAL
ISSN1530-437X
卷号23期号:16页码:18142-18149
通讯作者Shao, Congying(shaocongying@163.com) ; Lu, Shun(lushun@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:001049997900033
语种英语