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	Extracellular protein analysis of activated sludge and their functions in wastewater treatment plant by shotgun proteomics
	Zhang, Peng1; Shen, Yu2; Guo, Jin-Song1,2; Li, Chun1; Wang, Han1; Chen, You-Peng1,2; Yan, Peng2; Yang, Ji-Xiang2; Fang, Fang1
	2015-07-10
摘要	In this work, proteins in extracellular polymeric substances extracted from anaerobic, anoxic and aerobic sludges of wastewater treatment plant (WWTP) were analyzed to probe their origins and functions. Extracellular proteins in WWTP sludges were identified using shotgun proteomics, and 130, 108 and 114 proteins in anaerobic, anoxic and aerobic samples were classified, respectively. Most proteins originated from cell and cell part, and their most major molecular functions were catalytic activity and binding activity. The results exhibited that the main roles of extracellular proteins in activated sludges were multivalence cations and organic molecules binding, as well as in catalysis and degradation. The catalytic activity proteins were more widespread in anaerobic sludge compared with those in anoxic and aerobic sludges. The structure difference between anaerobic and aerobic sludges could be associated with their catalytic activities proteins. The results also put forward a relation between the macro characteristics of activated sludges and micro functions of extracellular proteins in biological wastewater treatment process.
DOI	10.1038/srep12041
URL	查看原文
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	5页码:11
通讯作者	Chen, YP (reprint author), Chongqing Univ, Key Lab Three Gorges Reservoir Reg Ecoenvironm MO, Chongqing 400045, Peoples R China.
收录类别	SCI
WOS记录号	WOS:000357715300001
语种	英语