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Bifunctional electrochemical biosensor based on PB-MXene films for the real-time analysis and detection of living cancer cells
Su, Xue1,2; You, Qiannan1,2; Zhuang, Linlin1,2; Chang, Zhimin2; Ge, Mingfeng2; Yang, Li3; Dong, Wen-Fei1,2
2023-09-20
摘要Circulating tumor cells (CTCs) are important prognostic markers for cancer diagnosis and metastasis, and their detection is an important means to detect cancer metastasis. Herein, we construct a novel bifunctional electrochemical biosensor based on the PB-MXene composite films. A simple electrostatic self-assembly approach was employed to prepare a film composed of PB nanocubes on the MXene substrates. Given that the PB is an artificial peroxidase for H2O2 sensing, the PB-MXene films can realize the real-time monitoring of H2O2 secretion from living CTCs. Besides, the anti-CEA attached biosensors can be utilized to quantify the corresponding CTCs. The synergic effects of the MXene with a large specific area and PB with enzyme-free catalysis for H2O2 resulted in PB-MXene films exhibiting high electrocatalytic and low cytotoxicity for both H2O2 sensing and living CTCs capturing. As a result, the biosensor shows a low detection limit of 0.57 & mu;M towards H2O2 with a wide linear range (1 & mu;M to 500 & mu;M), as well as an excellent sensing performance for CTCs (an extremely low detection limit of 9 cells/mL in a wide linear range of 1.3 x101 to 1.3 x106 cells/mL). Moreover, the prepared biosensor showed satisfactory stability and anti-interference ability for potential applications in clinical cancer diagnosis and tumor metastasis.
关键词Electrochemical biosensor MXene Prussian blue Living cancer cells
DOI10.1016/j.jpba.2023.115479
发表期刊JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
卷号234页码:9
通讯作者Yang, Li(ylyhp@126.com) ; Dong, Wen-Fei(wenfeidong@sibet.ac.cn)
收录类别SCI
WOS记录号WOS:001044767700001
语种英语