中文版 | English
CSpace
Copper oxidase and encapsulin shell protein in anammox bacteria reduces phenol toxicity from water
Ma, Teng-Fei1,4; Yu, Xiao-Yao1; Xing, Chong-Yang2,3; Shen, Yu1; Chen, You-Peng4; Chen, Xuan4; Peng, Meng-Wen4,5
2023-06-01
摘要The inhibition of phenol on anammox bacteria limits the application of anammox in the treatment of phenolcontaining wastewaters. The molecular mechanism of how anammox bacteria resist phenol toxicity in wastewater remains unclear. In this study, copper oxidase (Cop) and encapsulin shell protein (cEnc) from anammox bacteria Candidatus Brocadia fulgida were found to assist bacteria to resist phenol stress. The Cop and its complex with cEnc (cEnc-Cop) expressed in vitro by molecular biology had laccase-like activities (1.39 U/mg and 0.79 U/ mg, respectively). Combined with real-time quantitative polymerase chain reaction, both the Cop and cEnc gene expression levels were upregulated by stimulating anammox bacteria with phenol for a short time. It was also found that hydrazine synthase genes increased first and then decreased. These results indicated that cEnc-Cop relieved the inhibition of phenol to hydrazine synthase by degrading phenol, and consequently restored anammox activity. These findings will contribute to a deeper insight into the mechanism of how anammox bacteria respond to phenol and phenolic compound stress, and provide a theoretical reference for the application of anammox technology to phenol and phenolic compound-containing wastewater treatment.
关键词Anammox Encapsulin shell protein Copper oxidase Phenol
DOI10.1016/j.jece.2023.110297
发表期刊JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
ISSN2213-2929
卷号11期号:3页码:8
通讯作者Chen, You-Peng(ypchen@cqu.edu.cn)
收录类别SCI
WOS记录号WOS:001042262500001
语种英语