KMS Chongqing Institute of Green and Intelligent Technology, CAS
Bioflocculation characteristics of bound extracellular polymers substances from Pseudomonas sp. XD-3 and behavior of polysaccharides | |
An, Qiang1,2; Deng, Shu Man1,2; Zhao, Bin1,2; Huang, Chuang1,2; Yang, Ji Xiang3 | |
2023-08-01 | |
摘要 | This study aimed to investigate the bioflocculation characteristics of bound extracellular polymers substances (B-EPS), which were extracted from Pseudomonas sp. XD-3. The flocculation efficiency of B-EPS achieved about 80%-95% with an initial pH of 4-7, kaolin concentrations of 3-7 g L-1, temperature of 25-100 celcius and B-EPS dosage of 9-105 mg L-1. The bioflocculation process of B-EPS conformed to pseudo-second-order kinetic mode, suggesting that the bioflocculation belonged to chemical adsorption process. Enzymatic hydrolysis experiments demonstrated that both polysaccharides and proteins were active components for bioflocculation. The poly-saccharides were irregular aggregates with rough and porous surfaces and contained hydroxyl and carboxyl groups, which helped to promote bridging effect. Ribose, glucose and galactose were the main monosaccharides of polysaccharides. The molecular weight of the polysaccharides was relatively small, but the relatively loose configuration exposed more ion bridging sites, thus promoting the bioflocculation. Optimizing the ingredients of culture medium and culture time for B-EPS were effective strategies to increase the yield of flocculation active components. When the conditions were 10% of 2 g L-1 KH2PO4 + 5 g L-1 K2HPO4, 0.05% of Tween-80, citrate as carbon source and 32-48 h of culture time, both proteins and polysaccharides in B-EPS were significantly improved. This study gives an in-deep understanding on the flocculation characteristics of a novel bioflocculant from Pseudomonas sp. XD-3, which is conducive to the widespread application of bioflocculation. |
关键词 | Bioflocculant B-EPS Proteins Polysaccharides Bridging |
DOI | 10.1016/j.colsurfb.2023.113436 |
发表期刊 | COLLOIDS AND SURFACES B-BIOINTERFACES |
ISSN | 0927-7765 |
卷号 | 228页码:9 |
通讯作者 | Zhao, Bin(binzhao11@cqu.edu.cn) |
收录类别 | SCI |
WOS记录号 | WOS:001054505900001 |
语种 | 英语 |