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	Occurrence State and Molecular Structure Analysis of Extracellular Proteins with Implications on the Dewaterability of Waste-Activated Sludge
	Wu, Boran1; Ni, Bing-Jie1; Horvat, Kristine2; Song, Liyan3; Chai, Xiaoli1; Dai, Xiaohu1; Mahajan, Devinder2
	2017-08-15
摘要	The occurrence state and molecular structure of extracellular proteins were analyzed to reveal the influencing factors on the water-holding capacities of protein-like substances in waste-activated sludge (WAS). The gelation process of extracellular proteins verified that advanced oxidation processes (AOPs) for WAS dewaterability improvement eliminated the water affinity of exttacellular proteins and prevented these macromolecules from forming stable colloidal aggregates. Isobaric tags for relative and absolute quantitation proteomics identified that most of the extracellular proteins were originally derived from the intracellular part and the proteins originally located in the extracellular part were mainly membrane-associated. The main mechanism of extracellular protein transformation during AOPs could be represented by the damage of the membrane or related external encapsulating structure and the release of intracellular substances. For the selected representative extracellular proteins, the strong correlation (R-2 > 0.97, p < 0.03) between the surface hydrophilicity index and alpha-helix percentages in the secondary structure indicated that the water affinity relied more on the spatial distribution of hydrophilic functional groups rather than the content. Destructing the secondary structure represented by the alpha-helix and stretching the polypeptide aggregation in the water phase through disulfide bond removal might be the key to eliminating the inhibitory effects of extracellular proteins on the interstitial water removal from WAS.
DOI	10.1021/acs.est.7b02861
发表期刊	ENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN	0013-936X
卷号	51期号:16页码:9235-9243
通讯作者	Dai, XH (reprint author), Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, 1239 Siping Rd, Shanghai 200092, Peoples R China.
收录类别	SCI
WOS记录号	WOS:000407987400039
语种	英语