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Single-molecule lipopolysaccharides identification and the interplay with biomolecules via nanopore readout
Zhu, Rui1,2,3; Qin, Fupeng1; Zheng, Xinchuan1,2; Fang, Shaoxi1,2; Ding, Jianjun4; Wang, Deqiang1,2; Liang, Liyuan1,2
2023-11-15
摘要Lipopolysaccharides (LPS) are the major constituent on the cell envelope of all gram-negative bacteria. They are ubiquitous in air, and are toxic inflammatory stimulators for urinary disorders and sepsis. The reported optical, thermal, and electrochemical sensors via the intermolecular interplay of LPS with proteins and aptamers are generally complicated methods. We demonstrate the single-molecule nanopore approach for LPS identification in distinct bacteria as well as the serotypes discrimination. With a 4 nm nanopore, we achieve a detection limit of 10 ng/mL. Both the antibiotic polymyxin B (PMB) and DNA aptamer display specific binding to LPS. The identification of LPS in both human serum and tap water show good performance with nanopore platforms. Our work shows a highly-sensitive and easy-to-handle scheme for clinical and environmental biomarkers determi-nation and provides a promising screening tool for early warning of contamination in water and medical supplies.
关键词Solid-state nanopore Lipopolysaccharides Polymyxin B Aptamer Single-molecule
DOI10.1016/j.bios.2023.115641
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号240页码:8
通讯作者Wang, Deqiang(dqwang@cigit.ac.cn) ; Liang, Liyuan(liangliyuan@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:001069418400001
语种英语