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One-step competitive assay for detection of thrombin via disassembly of diblock oligonucleotide functionalised nanogold aggregates
Huang, Yu1,9; Li, Shengqiang2,3; Liu, Cuiying4; Chen, Ligang1; Qian, Husun5; Ho, Ho-Pui6; Wu, Jiangling7,8; Wu, Jiang1,9; Pu, Xiaoyin1,9
2023-02-01
摘要In this study, we developed a simple, sensitive and cost effective sensing strategy for thrombin. Two polyadenine diblock oligonucleotides, one containing a thrombin aptamer (TA) and the other one a partial complementary sequence to thrombin aptamer (TAC), were designed to anchor on gold nanoparticle (AuNP) surface. The hybridisation of these two DNA strands promoted the assembly of some AuNPs together and formed AuNP ag-gregates. The introduction of thrombin competitively bound to its aptamer and triggered the disassembly of AuNP aggregates. The change in plasmon absorption of AuNPs generated significant signal without requiring labeling or preprocessing steps. This simple one-step competitive assay had been demonstrated for the detection of thrombin with a detection limit of 0.81 pM and a dynamic range from 10 pM to 10 nM. Furthermore, this method was successfully demonstrated to perform analysis in 10-fold diluted serum solutions without sophis-ticated instruments. This sensitive and convenient strategy is expected to provide a prospective alternative in clinical application in a fast and straight forward manner.
关键词Surface plasmon resonance Gold nanoparticle aggregates Competitive bioassay Biosensing
DOI10.1016/j.snb.2022.133032
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号376页码:10
通讯作者Huang, Yu(huangyu@cigit.ac.cn) ; Wu, Jiangling(wujiangling@hospital.cqmu.edu.cn)
收录类别SCI
WOS记录号WOS:000904410500009
语种英语