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Alterations in immune cell heterogeneities in the brain of aged zebrafish using single-cell resolution
He, Jiangyong1,2; Zhao, Fangying1; Chen, Bingyue1; Cui, Nianfei1; Li, Zhifan1; Qin, Jie1; Luo, Lingfei1; Zhao, Congjian3; Li, Li1,2
2023-01-04
摘要Immunocytes, including the microglia, are crucial in the neurodegenerative process in old people. However, the understanding of regarding microglia heterogeneity and other involved immunocytes remains elusive. We analyzed 26,456 immunocytes from 12-and 26-month-old zebrafish brains at single-cell resolution. Microglia and T lymphocytes were detected in the brain at both time points. Two types of microglia were annotated, namely, ac(+) microglia and xr(+) microglia, which were clustered into subsets 1, 2, 3, 4, 5, and subsets 6, 7, 8, 9, respectively. Diversified microglia predominated the adult brains and cooperated with T cells to perform the functions of immune response and neuronal nutrition. We validated the specific microglia markers. The novel transgenic lines, Tg(lgals3bpb:eGFP) and Tg(apoc1:eGFP), were created, which faithfully labeled ac(+) microglia and served as valuable labeling tools. However, the microglia population reduced while T cells of six subtypes intriguingly increased to serve as the primary immune cells in aged brains. Unlike in 12-month-old brains, T cells, together with microglia, exhibited a coordinated signature of inflammation in the 26-month-old brains. Our findings revealed the immunocytes atlas in aged zebrafish brains. It implied the involvement of microglia and T cells in the progression of neurodegeneration in aging.
关键词aging scRNA-seq microglia T lymphocyte inflammation zebrafish
DOI10.1007/s11427-021-2223-4
发表期刊SCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
页码21
通讯作者Zhao, Congjian(cj.zhao@yahoo.com) ; Li, Li(lili@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000909434200001
语种英语