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DNA nanostructure-assisted detection of carcinoembryonic antigen with a solid-state nanopore
Tian, Rong1,2; Weng, Ting1,2; Chen, Shanchuan1,2; Wu, Ji1,2; Yin, Bohua1,2,3; Ma, Wenhao1,2; Liang, Liyuan1,2; Xie, Wanyi1,2; Wang, Yunjiao1,2; Zeng, Xiaoqing1,2,4
2023-02-01
摘要In this paper, a novel detection technique for tumor marker carcinoembryonic antigen (CEA) has been developed by using a solid-state nanopore as a tool. The system utilizes the specific affinity between aptamer-modified magnetic Fe3O4 and CEA, rather than directly detecting the translocation of CEA through the nanopore. The aptamer-modified magnetic Fe3O4 was hybridized with tetrahedral DNA nanostructures (TDNs), and TDNs were released after CEA was added. We investigate the translocation behavior of individual TDNs through solid-state nanopores. The frequency of the blockage signals for TDNs is recorded for indirect detection of CEA. We realized the detection of CEA with a concentration as low as 0.1 nM and proved the specificity of the interaction between the aptamer. In addition, our designed nanopore sensing strategy can detect CEA in real samples.
关键词Carcinoembryonic antigen Tetrahedral DNA Solid-state nanopore Aptamer
DOI10.1016/j.bioelechem.2022.108284
发表期刊BIOELECTROCHEMISTRY
ISSN1567-5394
卷号149页码:8
通讯作者Yin, Yajie(yinyajie@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000874821600004
语种英语