中国科学院重庆绿色智能技术研究院机构知识库

QQ客服 官方微博 反馈留言

	Proteomics reveals the enhancing mechanism for eliminating toxic hydroxylamine from water by nanocompartments containing hydroxylamine oxidase
	Xing, Chong -Yang1,2,3; Li, Guang-Yi4; Wang, Que1; Guo, Jin-Song1; Shen, Yu5; Yan, Peng1; Fang, Fang1; Chen, You -Peng1
	2022-10-15
摘要	Hydroxylamine (NH2OH) is a potentially toxic pollutant when it is present in water, as it can damage both bacteria and the human body. It is still difficult to eliminate the toxic NH2OH in water. Here, we showed that the model bacterium (Escherichia coli) with nanocompartments encapsulated with hydroxylamine oxidase (HAO) can remove NH2OH from water. In addition, the removal efficiency of NH2OH by genetically modified bacteria (with HAO-nanocompartments) was 3.87 mg N L-1 h(-1), and that of wild-type bacteria (without HAO-nanocompartments) was only 1.86 mg N L-1 h(-1). Label-free quantitative proteomics indicated that the nano -compartments containing HAO enhanced bacterial activity by inducing the up-regulation of proteins involved in stress and stimulus responses, and decreased their intracellular NH2OH concentration. Moreover, the synthesis of proteins involved in energy metabolism, gene expression, and other processes in bacterial was enhanced under hydroxylamine stress, and these changes increased the resistance of bacterial to NH2OH. This work can aid our understanding of the toxic effects of NH2OH on bacteria as well as the development of new approaches to eliminate NH2OH in water.
关键词	Hydroxylamine Proteomics Nanocompartments Hydroxylamine oxidase
DOI	10.1016/j.jhazmat.2022.129787
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
卷号	440页码:12
通讯作者	Chen, You -Peng(ypchen@cqu.edu.cn)
收录类别	SCI
WOS记录号	WOS:000860755500002
语种	英语