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Macrophages Rapidly Seal off the Punctured Zebrafish Larval Brain through a Vital Honeycomb Network Structure
Zou, Dandan1; Qin, Jie1; Hu, Wenlong1; Wei, Zongfang1; Zhan, Yandong1; He, Yuepeng1; Zhao, Congjian2; Li, Li3
2022-09-01
摘要There is accumulating evidence that macrophages play additional important roles in tissue damage besides their typical phagocytosis. Although the aggregation of macrophages on injured sites has long been observed, few researchers have focused on the role of the overall structure of macrophage aggregation. In this study, we developed a standardized traumatic brain injury (TBI) model in zebrafish larvae to mimic edema and brain tissue spillage symptoms after severe brain trauma. Using time-lapse imaging, we showed that macrophages/microglia in zebrafish larvae responded rapidly and dominated the surface of injured tissue, forming a meaningful honeycomb network structure through their compact aggregation and connection. Disrupting this structure led to fatal edema-like symptoms with severe loss of brain tissue. Using the RNA-Seq, together with the manipulation of in vitro cell lines, we found that collagen IV was indispensable to the formation of honeycomb network structures. Our study thus revealed a novel perspective regarding macrophages forming a protective compact structure with collagen IV. This honeycomb network structure acted as a physical barrier to prevent tissue loss and maintain brain homeostasis after TBI. This study may provide new evidence of macrophages' function for the rapid protection of brain tissue after brain injury.
关键词macrophages aggregation stab collagen TBI
DOI10.3390/ijms231810551
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号23期号:18页码:26
通讯作者Li, Li(lili@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000858605500001
语种英语