中文版 | English
CSpace
Exposure to Sri Lanka's local groundwater in a CKDu prevalent area causes kidney damage in zebrafish
Yang, Yi-Fan1,2; Li, Wei-Guo1; Wen, Ping-Ping1,2; Jia, Pan -Pan2; Li, Yong-Zhi3,4; Li, Tian-Yun3,4; Pei, De-Sheng2
2022-10-01
摘要How local groundwater induces chronic kidney disease of unknown etiology (CKDu) in Sri Lanka is still elusive. This study aims to elucidate the impacts of Sri Lanka's local groundwater in a CKDu prevalent area and reveal the possible pathogenic mechanism of CKDu using zebrafish models. The drinking water from the local underground well in Vavuniya was sampled and the water quality parameters including Na+, Mg2+, K+, Ca2+, Cl-, NO3-, SO42-, and F(- )were analyzed. Then, local groundwater exposure to zebrafish larvae and 293T cells was per-formed, and water with high hardness and fluoride was prepared as parallel groups. Our result showed that exposure to Sri Lanka's local groundwater caused developmental toxicity, kidney damage, and pronephric duct obstruction as well as abnormal behavior in zebrafish. Similar results were also found after exposure to water with high hardness and fluoride in zebrafish. Further, the expression levels of marker genes related to renal development and functions (foxj1a, dync2h1, pkd2, gata3, and slc20a1) were significantly altered, which is also confirmed in the 293T cells. Taken together, those results indicated that Sri Lanka's local groundwater in a CKDu prevalent area could cause kidney damage, implying that high water hardness and fluorine might be the inducible environmental factors for the etiological cause of CKDu.
关键词Local groundwater CKDU Water hardness Fluoride Kidney damage
DOI10.1016/j.aquatox.2022.106276
发表期刊AQUATIC TOXICOLOGY
ISSN0166-445X
卷号251页码:11
通讯作者Pei, De-Sheng(peids@cqmu.edu.cn)
收录类别SCI
WOS记录号WOS:000853356800008
语种英语