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Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(beta-amino ester)s
Li, Qiuxia1; Sun, Litao1; Huang, Xiaobei2; Liu, Shuai3; Yong, Haiyang1; Wang, Chenfei1; Li, Jianzhong4; Zhou, Dezhong1
2022-05-17
摘要Biodegradable and lipid-like highly branched poly(beta-amino ester)s, HPAESA, were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. Biodegradability reduces the cytotoxicity of HPAESA and enables controlled DNA release. Lipid mimicry enhances cellular uptake and endosomal escape of HPAESA/DNA polyplexes. HPAESA are able to transfect rat adipose-derived stem cells (rADSs) and human ADSCs (hADSCs) with orders of magnitude higher efficiency than commercial gene transfection reagents, with cell viability exceeding 90%. Most importantly, HPAESA can effectively transfer the nerve growth factor (NGF)-encoding plasmid to rADSCs and induce high NGF secretion, which significantly promotes neurite outgrowth of PC12 cells.
DOI10.1021/acsmacrolett.2c00095
发表期刊ACS MACRO LETTERS
卷号11期号:5页码:636-642
通讯作者Liu, Shuai(shuailiu@zju.edu.cn) ; Li, Jianzhong(jianzhong-0520@163.com) ; Zhou, Dezhong(dezhong.zhou@xjtu.edu.cn)
收录类别SCI
WOS记录号WOS:000804211500006
语种英语