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	Janus wireframe DNA cube-based 3D nanomachine for rapid and stable fluorescence detection of exosomal microRNA
	Xu, Yuan1,2,3,4; Li, Xinmin5,6; Niu, Changchun3; Wu, Haiping5; Yong, Yutao1,3; Qi, Caihong3; Gong, Wei5; Bai, Huijie5; Chen, Yirong5; Ding, Shijia5
	2022-09-15
摘要	Exosomal microRNAs (miRNAs) have been demonstrated to be credible biomarkers for the diagnosis and monitoring of tumors. Nevertheless, developing simple, rapid, and stable biosensing strategies that are capable of accurately detecting exosomal miRNAs remains a challenge. Herein, an accelerated and biostable threedimensional (3D) nanomachine based on Janus wireframe DNA cube was constructed for sensitive fluorescence measurement of exosomal miRNA. The Janus wireframe DNA cube could propel target exosomal miRNA21 rapid movement on its surface by catalytic hairpin assembly (CHA), releasing a massive fluorescence signal. Benefiting from the Janus wireframe DNA cube, the developed 3D nanomachine exhibited significantly improved reaction rate and enhanced biostability in complex biofluids compared to conventional CHA. As a result, this fluorescence biosensing strategy achieved rapid, stable, and single-step detection of exosomal miRNA-21 with the detection limit down to the picomole level. Therefore, this work offers a brief sensing tool for nucleic acid biomarkers detection, which has great application potential in tumor diagnosis.
关键词	Janus wireframe DNA cube Catalytic hairpin assembly DNA nanomachine Exosomal miRNA
DOI	10.1016/j.bios.2022.114405
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	212页码:8
通讯作者	Liao, Pu(liaopu@ucas.ac.cn)
收录类别	SCI
WOS记录号	WOS:000806013300003
语种	英语