中国科学院重庆绿色智能技术研究院机构知识库

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	Trapping and releasing of single microparticles and cells in a microfluidic chip
	Lv, Dan1; Zhang, Xiaoling2,3; Xu, Mengli1; Cao, Wenyue1; Liu, Xing1; Deng, Jinan1; Yang, Jun1; Hu, Ning1
	2022-07-03
摘要	A microfluidic device was designed and fabricated to capture single microparticles and cells by using hydrodynamic force and selectively release the microparticles and cells of interest via negative dielectrophoresis by activating selected individual microelectrodes. The trap microstructure was optimized based on numerical simulation of the electric field as well as the flow field. The capture and selective release functions of the device were verified by multi-types microparticles with different diameters and K562 cells. The capture efficiencies/release efficiencies were 95.55% +/- 0.43%/96.41% +/- 1.08% and 91.34% +/- 0.01%/93.67% +/- 0.36% for microparticles and cells, respectively. By including more traps and microelectrodes, the device can achieve high throughput and realize the visual separation of microparticles/cells of interest in a large number of particle/cell groups.
关键词	hydrodynamic trapping negative dielectrophoresis particle manipulation single-cell analysis single-cell releasing
DOI	10.1002/elps.202200091
发表期刊	ELECTROPHORESIS
ISSN	0173-0835
页码	10
通讯作者	Hu, Ning(huning@cqu.edu.cn)
收录类别	SCI
WOS记录号	WOS:000820116500001
语种	英语