KMS Chongqing Institute of Green and Intelligent Technology, CAS
Simultaneous fluorescent detection of multiple metal ions based on the DNAzymes and graphene oxide | |
Yun, Wen1; Wu, Hong1; Liu, Xingyan1; Fu, Min1; Jiang, Jiaolai4; Du, Yunfeng4; Yang, Lizhu2; Huang, Yu3 | |
2017-09-15 | |
摘要 | A novel fluorescent detection strategy for simultaneous detection of Cu2+, Pb2+ and Mg2+ based on DNAzyme branched junction structure with three kinds of DNAzymes and graphene oxide (GO) was presented. Three fluorophores labeled DNA sequences consisted with enzyme-strand (E-DNA) and substrate strand (S-DNA) were annealed to form DNAzyme branched junction structure. In the presence of target metal ion, the DNAzyme was activated to cleave the fluorophore labeled S-DNA. The S-DNA fragments were released and adsorbed onto GO surface to quench the fluorescent signal. The detection limit was calculated to be 1 nM for Cu2+, 200 nM for Mg2+, and 0.3 nM for Pb2+, respectively. This strategy was successfully used for simultaneous detection of Cu2+, Mg (2+) and Pb2+ in human serum. Moreover, it had potential application for simultaneous detection of multiple metal ions in environmental and biological samples. (C) 2017 Elsevier B.V. All rights reserved. |
关键词 | Dnazyme Graphene Oxide Metal Ions Fluorescent |
DOI | 10.1016/j.aca.2017.07.015 |
发表期刊 | ANALYTICA CHIMICA ACTA |
ISSN | 0003-2670 |
卷号 | 986页码:115-121 |
收录类别 | SCI |
WOS记录号 | WOS:000408863900012 |
语种 | 英语 |