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Tissue distribution and endocrine disruption effects of chronic exposure to pharmaceuticals and personal care products mixture at environmentally relevant concentrations in zebrafish
Hamid, Naima1,2,3; Junaid, Muhammad4; Manzoor, Rakia3,5; Duan, Jin-Jing1; Lv, Ming4; Xu, Nan4; Pei, De-Sheng1
2022
摘要Pharmaceuticals and personal care products (PPCPs) as emerging contaminants are ubiquitously present in the aquatic environment. Using in vivo and in silico techniques, this study aims to elucidate tissue distribution and endocrine disruption effects of chronic exposure (120 days) to PPCP mixture at environmentally relevant concentrations (ERCs) in adult zebrafish. Results from UHPLC-MS/MS analyses showed elevated distribution of PPCPs in zebrafish tissues in the order of liver > gonad > brain. Upregulation of steroid hormone receptors, both gonadotropin, and steroidogenic genes perturb the HPG axis pathway in females, while male fish exhibited significantly downregulated expressions of vtg, cyp17, and 17 beta hsd genes with inhibited fecundity. The Spearman correlation indicated a significant positive relationship between PPCPs bioaccumulation and mRNA levels of HPG axis genes. In silico molecular docking (MD) revealed specific amino acid residues of PPCPs binding with zebrafish estrogen receptors. Furthermore, the strongest binding energies of sulfamethoxazole, carbamazepine, and triclosan were discovered in er alpha and er beta estrogen receptors, confirming PPCPs' xenoestrogenic behavior. To summarize, chronic exposure to ERCs resulted in a high accumulation of PPCPs in the liver and gonad tissues of adult zebrafish, as well as associated perturbed genetic responses. As a result, strict environmental regulations for the disposal of PPCPs should be ensured to protect ecological and public health.
关键词PPCPs toxicity Xenoestrogens HPG axis Estrogen receptors Molecular docking
DOI10.1016/j.aquatox.2021.106040
发表期刊AQUATIC TOXICOLOGY
ISSN0166-445X
卷号242页码:11
通讯作者Xu, Nan(xunan@pkusz.edu.cn) ; Pei, De-Sheng(peids@cqmu.edu.cn)
收录类别SCI
WOS记录号WOS:000728557800001
语种英语