KMS Chongqing Institute of Green and Intelligent Technology, CAS
An electrochemical aptasensor for highly sensitive detection of CEA based on exonuclease III and hybrid chain reaction dual signal amplification | |
Niu, Cui1; Lin, Xiaojuan1; Jiang, Xin2; Guo, Fei3; Liu, Jianxiao1; Liu, Xiangqin1; Huang, Hongge1; Huang, Yu4,5 | |
2022-02-01 | |
摘要 | At present, carcinoembryonic antigen (CEA) is considered a broad-spectrum cancer biomarker, and its accurate analysis in clinical samples can assist early cancer diagnosis and treatment. Herein, a novel electrochemical aptasensor has been proposed for CEA detection based on exonuclease III and hybrid chain reaction. The target CEA specifically binds to the aptamer region in hairpin probe 1 (defined as H1) by strong attraction, which leads the rest of the H1 triggering catalytic hairpin assembly to form a high quantity of H1 and hairpin probe 2 (defined as H2) double chain complex (denoted as H1@H2). Subsequently, the exonuclease III digests the complex of H1@H2 and liberates H1 to induce the first signal amplification. Simultaneously, a large number of generated trigger chains initiate a hybrid chain reaction and produce a second signal amplification. This proposed sensor exhibited excellent analytical performance for the detection of CEA, with wide linear range from 10 pg.mL(-1) to 100 ng.mL(-1) and low limit of detection of 0.84 pg.mL(-1). Additionally, the biosensing strategy was successfully verified for direct measurement of CEA in human serum. Therefore, this elaborated sensor provides a new simple method for detecting CEA and exhibits great promise in the early screening of cancer. (C) 2021 Elsevier B.V. All rights reserved. |
关键词 | Carcinoembryonic antigen Electrochemical aptasensor Exonuclease III Hybrid chain reaction Signal amplifification |
DOI | 10.1016/j.bioelechem.2021.107986 |
发表期刊 | BIOELECTROCHEMISTRY |
ISSN | 1567-5394 |
卷号 | 143页码:7 |
通讯作者 | Huang, Yu(huangyu@cigit.ac.cn) |
收录类别 | SCI |
WOS记录号 | WOS:000719358200005 |
语种 | 英语 |