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Enzyme Hinders HIV-1 Tat Viral Transport and Real-Time Measured with Nanopores
Wang, Han1,2,3; Huang, Wenli4; Wang, Yunjiao1,2; Li, Wei1,2; Liu, Qianshan1,2; Yin, Bohua1; Liang, Liyuan1,2; Wang, Deqiang1,2; Guan, Xiyun4; Wang, Liang1,2,3
2021-10-22
摘要HIV-1 Tat protein, an intercellular transporter with a determinant function of delivering "information-rich" molecules in viral multiplication, was tryptic-hydrolyzed and real-time single molecule-monitored in a transmembrane pore. The electrokinetic studies revealed the catalytic and inhibitory effects on enzymatic digestion associated with Ca2+ and Cu2+ ions, respectively, in response to binding interactions with trypsin. Our strategy permits accurate and distinguishable sensing of Ca2+ and Cu2+ via an enzyme assay. In addition, considering the closer mimic of the real situation of HIV spread, measurements in the serum and on cells were also investigated. Transmembrane current measurements together with fluorescence microscopy imaging indicated the potential to perturb the Tat transport in the serum environment and on cells. Because the involved Tat proteolysis should prevent the occurrence of viral delivery, the presented method probably enables efficient hindrance to HIV-1 infection, in complementary to current traditional treatments.
关键词transmembrane pore HIV-1 Tat protein enzymatic proteolysis copper calcium molecular transport
DOI10.1021/acssensors.1c01717
发表期刊ACS SENSORS
ISSN2379-3694
卷号6期号:10页码:3781-3788
通讯作者Guan, Xiyun(xguan5@iit.edu) ; Wang, Liang(wangliang83@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000711034500030
语种英语