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Nanopore detects.-radiation inhibited HIV-1 protease activity
Li, Minghan1,2,3; Li, Wei2,3; Xiao, Yicen2,3; Liu, Qianshan2,3; Liang, Liyuan2,3; Wang, Deqiang2,3; Huang, Wenli4; Wang, Liang2,3,5
2021-12-15
摘要Inhibition of HIV-1 protease (PR) activity is realized by exposure to Co-60 gamma-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows similar to 50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of gamma-radiation (0.1K Gy), and the values reach to over 90% with high gamma-ray treatment. Besides, the inactivation effect is also verified in blood samples which contain the virus protease. Our finding provides the potential benefits of gamma-radiation to inactivate viral proteinic function, and might be a complementary to the designation of HIV-1 PR inhibitors.
关键词HIV-1 protease GagPol precursor Enzyme inactivation Co-60 gamma-radiation Nanopore
DOI10.1016/j.bios.2021.113602
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号194页码:5
通讯作者Huang, Wenli(wenlih11@126.com) ; Wang, Liang(wangliang83@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000697620500002
语种英语