中国科学院重庆绿色智能技术研究院机构知识库

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	Generation of a novel transgenic marine medaka (Oryzias melastigma) for highly sensitive detection of heavy metals in the environment
	Chen, Ya-Nan1,2; Bian, Wan-Ping2; Liu, Li2; Chen, Xin1; Tang, Min1; Pei, De-Sheng2,3
	2021-10-05
摘要	As typic priority pollutants in the marine environment, heavy metals can be accumulated in the human body leading to serious environmental and health problems. The metal regulatory elements (MREs) have been identified to be the main functional parts for the response to heavy metals. To develop a convenient biological monitoring tool for the detection of heavy metals in the oceans, we generated a transgenic marine medaka line Tg (OmMT: eGFP) with a truncated metallothionein promoter, which was only 193 bp and drove the expression of eGFP. After Tg(OmMT:eGFP) embryos were treated with four different heavy metals and different concentrations, the results showed that the expression level of eGFP was consistent with that of the endogenous mt. The trans genic embryos are very sensitive to Hg2+, and the fluorescence could be induced in the 0.0002 mu M concentration, which is far lower than the primary water standard. The expression level of eGFP and mt showed a dose-dependent manner to heavy metals concentration. Taken together, the newly established marine medaka is a sensitive, efficient, and convenient tool for monitoring heavy metal pollution in the environment, especially seawater.
关键词	Oryzias melastigma Metallothionein (mt) Promoter Heavy metals Transgenic marine medaka
DOI	10.1016/j.jhazmat.2021.126382
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
卷号	419页码:8
通讯作者	Tang, Min(tangmin@hainu.edu.cn) ; Pei, De-Sheng(peids@cigit.ac.cn)
收录类别	SCI
WOS记录号	WOS:000696614600001
语种	英语