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Nanopore Technology for the Application of Protein Detection
Zeng, Xiaoqing1,2,3; Xiang, Yang1,2,3; Liu, Qianshan2,3; Wang, Liang2,3; Ma, Qianyun2,3,4; Ma, Wenhao1,2,3; Zeng, Delin2,3,5; Yin, Yajie2,3; Wang, Deqiang2,3
2021-08-01
摘要Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated.
关键词nanopore single-molecule detection protein detection solid-state nanopore
DOI10.3390/nano11081942
发表期刊NANOMATERIALS
卷号11期号:8页码:16
通讯作者Yin, Yajie(yinyajie@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000689865800001
语种英语