KMS Chongqing Institute of Green and Intelligent Technology, CAS
Cross Disjoint Mortise Confined Solid-State Nanopores for Single-Molecule Detection | |
Liu, Yexiang1,2; Zhang, Xiaoling1,2; Xie, Wanyi1,2; Fang, Shaoxi1,2; Yin, Bohua1,2; Zhou, Daming1,2; Tang, Jing1,2; Liang, Liyuan1,2; Lu, Wenqiang1,2; He, Shixuan1,2 | |
2021-09-24 | |
摘要 | Solid-state nanopores have attracted widespread attention in the single-molecule detection application. However, getting controllable nanopores with high sensitivity and robustness requires a revolutionary breakthrough in nanopore fabrication. As a convenient and low-cost nanopore fabrication method, the controlled dielectric breakdown technology can hardly control the position and number of nanopores. This work proposes a concept of cross disjoint mortise confined solid-state nanopore (CDM-Nanopore) fabricated using a focused gallium ion beam and controlled dielectric breakdown technologies. A confined domain formed by the two cross disjoint mortise structures localizes the position for nanopore fabrication with a controlled dielectric breakdown method. The ion translocation analyses and noise performance of CDM-Nanopore were experimentally demonstrated with excellent sensitivities for long-term molecular detection due to their robustness and low noise characteristics of cross disjoint mortise structures. The surface charges have a great effect on the capture rate for DNA translocation in the confined nanopore while adding the formamide into G-quadruplex translocation experimentally. Besides, the COMSOL simulation results verify that the CDM-Nanopore has the same electrical properties as the conventional nanopore with the same effective thickness. These provide insight into an understanding of DNA translocation in the CDM-Nanopore to enable the localized nanopore fabrication with the controlled dielectric breakdown technology. Our results can be used to expand the membrane nanopores to zero-depth nanopores for molecular sensing and DNA/RNA sequencing. |
关键词 | solid-state nanopore controlled dielectric breakdown cross disjoint mortise structure CDM-Nanopore confined domain |
DOI | 10.1021/acsanm.1c02227 |
发表期刊 | ACS APPLIED NANO MATERIALS |
ISSN | 2574-0970 |
卷号 | 4期号:9页码:9811-9820 |
通讯作者 | He, Shixuan(heshixuan@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn) |
收录类别 | SCI |
WOS记录号 | WOS:000702079900116 |
语种 | 英语 |